RNA

Part:BBa_K3249001:Design

Designed by: Omar El-Fahmawi   Group: iGEM19_UC_Davis   (2019-10-16)


CHO IL1RN sgRNA 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NCBI’s gene search was used to find the sequence of the promoter region for IL1RN. Chop Chop (https://chopchop.cbu.uib.no/) was used to design the sgRNA sequence.

Source

Oligos were synthesized by IDT.

References